Ampicillin resistance gene sequence pbr322

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Register Already have an account? This vector is NOT available from Addgene. The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases through the Tet R gene.

Also, from Bolivar et al. In calculating the moving wall, the current year is not counted. This article has been cited by other articles in PMC. You can always find the topics here!

The Amp R gene is penicillin beta-lactamase. The "moving wall" represents the time period between the last issue available in JSTOR and the most recently published issue of a journal. Reviews and Protocols 2nd ed. Please review our privacy policy. Export a Text file For BibTex. The mapping and sequence determination of the single site in phiXam3 replicative form DNA cleaved by restriction endonuclease Pst I.

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The circular sequence is numbered such that 0 is the middle of the unique EcoRI site and the count increases first through the tet genes, the pMB1 material, and finally through the Tn3 region. Ampicillin-resistant derivatives of the plasmid pMB9".

This page is informational only. Many other plasmids were artificially constructed to create one that would be ideal for cloning purpose, and pBR was found to be most versatile by many and was therefore the one most popularly used. Add up to 3 free items to your shelf.

Origin of the TEM-beta-lactamase gene found on plasmids. From Wikipedia, the free encyclopedia. M Twyman 17 January Were these topics helpful? The plasmid has unique restriction sites for more than forty restriction enzymes.

National Academy of Sciences. This is a free resource for the scientific community that is compiled by Addgene. Abstract I have determined the nucleotide sequence of the ampicillin resistance gene of pBR, an Escherichia coli plasmid that encodes a penicillin beta-lactamase.

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This gene codes for a protein of amino acid residues. Promoter P3 is the natural promoter for the beta-lactamase gene.

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