Assay of clopidogrel
National Center for Biotechnology InformationU. Solid line represents bias 6. Partial correlation controlling for the randomization group between aggregation results obtained by the various platelet function assays.
AddSuppFiles-2 - ppt file. Sonicate for 5 minutes, and stir with a magnetic stirrer for 30 minutes. However, our results are in agreement with several reports describing its inability to monitor clopidogrel therapy. Ultrarapid metabolizers may be at increased risk of bleeding. Causes for Rejection Frozen or hemolyzed specimen; quantity not sufficient for analysis; one buccal swab; improper container; wet buccal swab.
Different methodologies for evaluating the effect of clopidogrel on platelet function in high-risk coronary artery disease patients. Advances in Cardiac Ultrasound, Davos A head-to-head comparison between the VerifyNow P2Y12 assay and light transmittance aggregometry for monitoring the individual platelet response to clopidogrel in patients undergoing elective percutaneous coronary intervention.
Bland—Altman analysis of agreement to assess how closely two measurements are related to each other, with limits of agreement depicting the range of variability in agreement between the two measures.
Didn't get the message? Large prospective clinical trials are necessary to determine the clinical value of platelet function tests, and their use in a clinical setting should therefore be avoided as long as the results of such studies are pending.
Two tubes must be collected.
Calculate the percentage of related compounds in the portion of Tablets taken by the formula: However, little is known about the comparability or interchangeability of these tests. Unlike warfarin, where measuring international normalized ratio can help direct management, measuring platelet inhibition from clopidogrel Plavix is not standardized.
To continue reading this article, you must log in with your personal, hospital, or group practice subscription. This has been hypothesized to be attributable, in part, to variable pharmacodynamic efficacy of one or both agents.
Please note that Internet Explorer version 8. We investigated the comparability of platelet function tests in quantifying platelet inhibition achieved by clopidogrel.
Even though the assay has become better automated, it remains time- and labour-intensive, requires technical expertise, and thus is restricted to specialized laboratories. Prasugrel achieves greater and faster P2Y 12 receptor-mediated platelet inhibition than clopidogrel due to more efficient generation of its active metabolite in aspirin-treated patients with coronary artery disease. Analyses were performed with SPSS Preoperative management of patients who are taking Plavix and require an invasive procedure or surgery can be problematic.
These patients may be clopidogrel nonresponders or noncompliant. We wish to acknowledge the technical assistance of our laboratory technician, Edmond Sia, as well as the assistance of our research nurse, Chantale Mercure, in the recruitment and blood sampling of the patients.
Although clopidogrel metabolites were not measured to ensure that patients took clopidogrel, compliance was verified by pill count and personal interview.